Research Journal of Engineering and Technology

M. Anil Kumar ¹, S. Keerthana ¹, Adyasa Pani ¹, D. Suresh ¹, M. Seenuvasan ¹

Affiliations
1 Department of Biotechnology, Madha Engineering College, Chennai, IN

Abstract

The combined effects of initial sucrose and initial Reactive Red C2G 29 dye concentrations on the specific growth rate and dye bioaccumulation efficiency of Penicillium chrysogenum MTCC 6477 was determined at pH 5.0. The highest growth rate of 0.115 h^-1 was obtained at an initial sucrose concentration of 15 g/L in absence of dye. For each constant sucrose concentration chosen between 5 and 15 g/L with an increase in initial dye concentration up to 500 mg/L under pH 5.0 resulted in a decrease in percentage uptake of dye and more over all dye concentration tested inhibited the growth of Penicillium chrysogenum. On the other hand at each constant dye concentration studied, both the specific growth rate and uptake yield enhanced with raising sucrose concentration up to15 g/L with pH 5.0, the maximum uptake yield of 96.67 % was observed in 15g/L sucrose and 50 mg/L dye containing growth medium at pH 5.0. To check the stability of this bioaccumulation process a continuous bioaccumulation system was developed and it shows monotonic damping oscillation where the dye uptake concentration approaches equilibrium.

Keywords

Penicillium chrysogenum, Mycelium, Bioaccumulation, Stability, Reactive Red.

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